The present study evaluated the effect of operating light exposure conditions on the shear bond strength (SBS) of composite resin bonded to enamel with three different universal adhesives used in self-etching mode. Tested adhesives were Single Bond Universal (SBU), G-Premio Bond (GB) and Nova Compo-B Plus (NB) and tested illumination conditions were ambient light, 22.000 lux, and 8.000 lux. Ninety bovine incisors were used for SBS test (n = 10). Effect of operating light exposure on the fluidity of adhesives was assessed. Resin-enamel interfaces were also evaluated under a scanning electron microscope. Two-way analysis of variance showed that material had a significant effect on SBS (p < 0.000) unlike the illumination condition (p = 0.459). GB provided significantly lower bond strength than those of SBU and NB (p < 0.05). High power operating light likely reduces the fluidity of all adhesives. Thus, it could be recommended that clinicians would prefer to use the tested resin adhesive systems at an illuminance of 8.000 lux.