This paper presents the results of karyological analysis of five Alchemilla species collected from northeast Anatolia, Turkey, belonging to sect. Alchemilla Rothm. subsect. Chirophyllum Rothm. ser. Sericeae Bus., subsect. Heliodrosium Rothm. ser. Vulgares Bus. and subsect. Calycanthum Rothm. ser. Elatae Rothm. The following chromosome numbers were determined: A. amoena (Czeczott) Rothm. 2n = 90-102, A. chlorosericea Bus. 2n = 92-103, A. glabricaulis Lindb. 2n = 90-109, A. hessii Rothm. 2n = 88-96, A. porrectidens Juz. 2n = 86-96. The chromosome numbers of three of these five species are presented for the first time.