Phenolic Profiles, Antimicrobial and Cytotoxic Properties of Both Micropropagated and Naturally Growing Plantlets of Calamintha sylvatica subsp. sylvatica Bromf.

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CÜCE M., BEKİRCAN T., Laghari A. H. , Sokmen M. , Sokmen A. , Ucar E. O. , ...More

NOTULAE BOTANICAE HORTI AGROBOTANICI CLUJ-NAPOCA, vol.47, no.4, pp.1145-1152, 2019 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 47 Issue: 4
  • Publication Date: 2019
  • Doi Number: 10.15835/nbha47411474
  • Page Numbers: pp.1145-1152
  • Keywords: antimicrobial activity, Calamintha sylvatica, cytotoxic activity, micropropagation, rosmarinic acid, HAIRY ROOT CULTURES, ROSMARINIC ACID, ESSENTIAL OILS, ANTIOXIDANT PROPERTIES, SUSPENSION-CULTURES, SOLVENT-EXTRACTION, LAMIACEAE, GROWTH, PROLIFERATION, L.


A rapid micropropagation protocol was designed to produce Calamintha sylvatica plantlets by using nodal segments as explants for the shoot formation. 6-BA favored the highest shoot formation and biomass yield, whilst kinetin was found superior for the highest shoot length (38.97 +/- 2.85 mm) and node numbers (2.89 +/- 0.63). Rosmarinic acid was detected as major phenolic acid, ranging from 7.59 mg/100 g to 81.44 mg/100 g. Hexane extracts from natural and in vitro propagated plantlets showed activity only against Staphylococcus aureus ATCC 25923 with MIC values at 6.25 and 3.33 m/mL, respectively while in the latter case, extracts from natural plantlets exerted higher cytotoxic activity than those of micropropagated ones (IC50 values were 83 mu g/mL and 98 mu g/mL on HeLa cells, respectively). C. sylvatica showed high micropropagation performance and produced remarkable amount of rosmarinic acid in vitro as well as antimicrobial and cytotoxic effect.