Akademik Veri Yönetim Sistemi
Federation of European Neuroscience Societies (FENS) 2024 Forum of Neuroscience, Vienna, Avusturya, 25 - 29 Haziran 2024, ss.3
The effect of living conditions on the physiology, behaviour and stress of laboratory animals is still being discussed. There are conflicting findings regarding individually ventilated cages(IVC). This study aims to explore the effects of three distinct cage setups and to examine the differences in the impacts of IVC cages, classical cages and classical cages with varying mouse populations.
Thirty male C57BL/6J strain mice were split into three groups:IVC, classical small(CC500) and big(CC800) with a floor size of 500 and 800 cm2,respectively. From birth until six months of age, the mice were placed in one of the cages. Body weights were measured. Hang wire and adhesive removal tests were used to assess somatosensory and motor skills. The forced swim test indicated the severity of depression-like behaviour. Video recordings of tests were made offline to confirm all scoring and analysis. Blood corticosterone levels were measured, as were brain malondialdehyde levels, total antioxidant status and total oxidant status. Statistical analysis was performed by one-way ANOVA.
In the adhesive removal test, the CC500 group performed lower than the others (p<0.001) and had the highest oxidative stress parameters (p<0.0001). The CC800 group had significantly increased body weight (p<0.01) and plasma corticosterone levels (p<0.001). There were no significant differences between the groups in the hang wire test and forced swim test.
According to our findings, the least stressful living condition was IVC cage. The number of mice housed in the classical cages was found to have a substantial impact on both stress levels and sensory-motor function.