Campanula latifolia L. subsp. latifolia'den Elde Edilen Ekstraktların Uçucu ve Fenolik Bileşenler ve Antioksidan, Asetilkolinesteraz, Tirozinaz, α- Glukozidaz İnhibitör Etkileri


Korkmaz B. , Fandaklı S., Yıldırım S. , Şener S. Ö. , Öztürk E. , Terzioğlu S. , ...Daha Fazla

Journal Of Essential Oil Bearing Plants, cilt.23, ss.1118-1131, 2020 (SCI Expanded İndekslerine Giren Dergi)

  • Cilt numarası: 23 Konu: 5
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1080/0972060x.2020.1856004
  • Dergi Adı: Journal Of Essential Oil Bearing Plants
  • Sayfa Sayıları: ss.1118-1131

Özet

This research aimed to investigate the effect of different extraction methods and chemical

composition of the essential oil (EO) and solid-phase microextraction (SPME) from Campanula latifolia L. subsp.

latifolia which is native to Turkey, with different medicinal properties. Phenolic components, antioxidant

and enzyme inhibitory effects of essential oil (EO), n-hexane, acetonitrile, methanol and water extracts of C. latifolia

subsp. latifolia were also studied. The volatile components of the EO, SPME and SPME of n-hexane extracts,

obtained from the Campanula latifolia L. subsp. latifolia, were analyzed by GC-FID/MS. A total of 32,

31 and 36 compounds were identified comprising 99.8 %, 99.9 % and 94.4 % of the SPME, SPME of n-hexane extract

and EO of C. latifolia subsp. latifolia, respectively. The major compounds identified in the SPME and SPME

of n-hexane extract were limonene (44.0 %) and 1,2,3-trimethyl benzene (27.4 %), respectively. Whereas the

EO of Campanula latifolia subsp. latifolia was rich with capro aldehyde (23.1 %), nonanal (9.3 %), 2(E)-hexenal

(6.8 %), benzene acetaldehyde (6.8 %), E-β-caryophyllene (4.4 %) and E-β-ionone (3.3 %). The phenolic content

of extracts was analyzed by RP-HPLC. Results revealed the sinapic, benzoic, syringic and p-coumaric acids as

major constituents. Antioxidant activities (DPPH, PRAP and FRAP) and enzyme inhibitory (tyrosinase, acetylcholinesterase

(AChE) and α-glucosidase) effects of the EO, n-hexane, acetonitrile, methanol and water extracts obtained

from C. latifolia subsp. latifolia were investigated. The acetonitrile extract, among the other extracts of

C. latifolia subsp. latifolia, was found to have the highest antioxidant activity and inhibitory effect for all tested

enzymes due to its high phenolic content.