Nonperipherally and peripherally substituted water-soluble magnesium (II) phthalocyanines and their DNA binding, nuclease activities

BARUT B., SEYHAN G., KELEŞ T., Kulein B., BIYIKLIOĞLU Z.

APPLIED ORGANOMETALLIC CHEMISTRY, cilt.38, sa.5, 2024 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 38 Sayı: 5
  • Basım Tarihi: 2024
  • Doi Numarası: 10.1002/aoc.7421
  • Dergi Adı: APPLIED ORGANOMETALLIC CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, BIOSIS, Chemical Abstracts Core, Chimica, Communication Abstracts, Compendex, Metadex, DIALNET, Civil Engineering Abstracts
  • Anahtar Kelimeler: magnesium, photonuclease, phthalocyanine, plasmid DNA, synthesis
  • Karadeniz Teknik Üniversitesi Adresli: Evet

Özet

In this study, magnesium (II) phthalocyanines (2a, 3a) and their water-soluble derivatives (2b, 3b) were synthesized via multistep reactions. The structures of these phthalocyanines were identified by FT-IR, NMR, MALDI-TOF, and UV-Vis spectroscopy. The ct-DNA binding (UV-Vis absorption, competitive EB binding, and agarose electrophoresis studies) and supercoiled plasmid DNA nuclease properties (hydrolytic, photonuclease, oxidative nuclease, and photooxidative nuclease) of the water-soluble compounds were investigated using different methods. The DNA binding constant (Kb) values of 2b and 3b were calculated as 8.45 +/- (0.25) x 104 and 7.71 +/- (0.13) x 104 M-1 at 25 degrees C, respectively. The results showed that 2b had a stronger ct-DNA binding effect than 3b according to Kb and r values. The DNA nuclease studies claimed that both compounds indicated photonuclease activity on plasmid DNA depending on the light dose. Additionally, 2b had a higher photonuclease capacity than 3b. All of these results showed that 2b had promising potential as a photosensitizer agent for photodynamic therapy. We have synthesized magnesium (II) phthalocyanines (2a, 3a) and their water-soluble derivatives (2b, 3b). The ct-DNA binding and supercoiled plasmid DNA nuclease properties of the water-soluble compounds were investigated using different methods. All of these results showed that 2b had promising potential as a photosensitizer agent for photodynamic therapy. image