In this study, we investigated the bacterial pathogens of Euproctis chrysorrhoea and tested for their insecticidal activities. Based on colony color and morphology, four isolates were determined. According to morphological, physiological and biochemical characters of the isolates, they were identified as Enterobacter aerogenes, Lactobacillus sp., Bacillus thuringiensis and Micrococcus luteus. The insecticidal effects of these bacterial isolates on third-fourth instar larvae of Euproctis chrysorrhoea were investigated. The highest insecticidal effect determined on this pest is 68% with Bacillus thuringiensis. The effects of the other isolates are 45% with Enterobacter aerogenes, 15% Micrococcus luteus and 0% with Lactobacillus sp.