Akademik Veri Yönetim Sistemi
7th EURASIA BIOCHEMICAL APPROACHES & TECHNOLOGIES (EBAT) CONGRESS, Antalya, Türkiye, 6 - 09 Kasım 2025, ss.32, (Özet Bildiri)
In this study, bioactive fractions were isolated from Mnium spinulosum and evaluated as a potential natural source of α-glucosidase inhibitor, widely used in the management of postprandial hyperglycemia1 . Dried plant material was powdered and extracted by Soxhlet using n-hexane/ethyl acetate (85:15, v/v), yielding a crude extract. The extract was subsequently fractionated via Silica gel column chromatography with a gradient elution of n-hexane/ethyl acetate mixtures. The resulting fractions were screened for α-glucosidase inhibitory activity in vitro. Among them, fraction fr3.1 showed the strongest inhibitory effect. Thin-layer chromatography (TLC) analysis of fr3.1 produced four isolates (izo1–izo4), of which izo2 exhibited the highest enzyme activity. The acute oral toxicity of izo2 was evaluated in male C57BL/6 mice according to OECD 425 guidelines. At a dose of 17 mg/kg, histopathological examination of liver, kidney, heart, and pancreas revealed no abnormalities, confirming safety at this concentration. However, administration of higher doses resulted in pathological changes, including vascular congestion, inflammation, degeneration, and edema. Overall, these findings identify izo2 from M. spinulosum as a bioactive natural compound with a significant α-glucosidase inhibitory potential and support its promise as a leading candidate for further antidiabetic drug development. This study was financially supported by TUBITAK (Project Number: 123Z349).