Bioactivity-guided isolation and quantification of Chlorogenic acid from Calystegia silvatica (Kit.) Griseb. (Convolvulaceae)
International Journal of Secondary Metabolite, cilt.12, sa.1, ss.158-165, 2025 (Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 12 Sayı: 1
- Basım Tarihi: 2025
- Doi Numarası: 10.21448/ijsm.1451604
- Dergi Adı: International Journal of Secondary Metabolite
- Derginin Tarandığı İndeksler: Scopus, CAB Abstracts, Central & Eastern European Academic Source (CEEAS), Directory of Open Access Journals, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.158-165
- Anahtar Kelimeler: Calystegia silvatica, Chlorogenic acid, Cytotoxic activity, HPLC-DAD, Resazurin assay
- Karadeniz Teknik Üniversitesi Adresli: Evet
Özet
This study aimed to investigate the bioactivity-guided isolation and quantification of chlorogenic acid from Calystegia silvatica, a plant traditionally utilized for its medicinal properties. The most cytotoxic aqueous sub-extract was subjected to open column chromatography to assess its cytotoxic activity against MCF-7 breast cancer cells using a resazurin reduction assay. The structure of the most active pure compound was determined to be chlorogenic acid (IC50= 36.44 ± 2.18 μg/mL) using 1D and 2D NMR spectroscopy. A validated high-performance liquid chromatography-diode array detector (HPLC-DAD) method was employed for the quantification of chlorogenic acid, which resulted in a content of 10.05 mg/g crude extract. The results indicate the potential of Calystegia silvatica as a source of chlorogenic acid with cytotoxic activity.