Bioactivity-guided isolation and quantification of Chlorogenic acid from Calystegia silvatica (Kit.) Griseb. (Convolvulaceae)

Baran M. Y., Şener S. Ö., KANBOLAT Ş., BADEM M., ÖZGEN U.

International Journal of Secondary Metabolite, cilt.12, sa.1, ss.158-165, 2025 (Scopus) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 12 Sayı: 1
  • Basım Tarihi: 2025
  • Doi Numarası: 10.21448/ijsm.1451604
  • Dergi Adı: International Journal of Secondary Metabolite
  • Derginin Tarandığı İndeksler: Scopus, CAB Abstracts, Central & Eastern European Academic Source (CEEAS), Directory of Open Access Journals, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.158-165
  • Anahtar Kelimeler: Calystegia silvatica, Chlorogenic acid, Cytotoxic activity, HPLC-DAD, Resazurin assay
  • Karadeniz Teknik Üniversitesi Adresli: Evet

Özet

This study aimed to investigate the bioactivity-guided isolation and quantification of chlorogenic acid from Calystegia silvatica, a plant traditionally utilized for its medicinal properties. The most cytotoxic aqueous sub-extract was subjected to open column chromatography to assess its cytotoxic activity against MCF-7 breast cancer cells using a resazurin reduction assay. The structure of the most active pure compound was determined to be chlorogenic acid (IC50= 36.44 ± 2.18 μg/mL) using 1D and 2D NMR spectroscopy. A validated high-performance liquid chromatography-diode array detector (HPLC-DAD) method was employed for the quantification of chlorogenic acid, which resulted in a content of 10.05 mg/g crude extract. The results indicate the potential of Calystegia silvatica as a source of chlorogenic acid with cytotoxic activity.