Bioactivity-guided isolation and quantification of Chlorogenic acid from Calystegia silvatica (Kit.) Griseb. (Convolvulaceae)

Baran M. Y., Şener S. Ö., KANBOLAT Ş., BADEM M., ÖZGEN U.

International Journal of Secondary Metabolite, vol.12, no.1, pp.158-165, 2025 (Scopus) identifier

  • Publication Type: Article / Article
  • Volume: 12 Issue: 1
  • Publication Date: 2025
  • Doi Number: 10.21448/ijsm.1451604
  • Journal Name: International Journal of Secondary Metabolite
  • Journal Indexes: Scopus, CAB Abstracts, Central & Eastern European Academic Source (CEEAS), Directory of Open Access Journals, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.158-165
  • Keywords: Calystegia silvatica, Chlorogenic acid, Cytotoxic activity, HPLC-DAD, Resazurin assay
  • Karadeniz Technical University Affiliated: Yes

Abstract

This study aimed to investigate the bioactivity-guided isolation and quantification of chlorogenic acid from Calystegia silvatica, a plant traditionally utilized for its medicinal properties. The most cytotoxic aqueous sub-extract was subjected to open column chromatography to assess its cytotoxic activity against MCF-7 breast cancer cells using a resazurin reduction assay. The structure of the most active pure compound was determined to be chlorogenic acid (IC50= 36.44 ± 2.18 μg/mL) using 1D and 2D NMR spectroscopy. A validated high-performance liquid chromatography-diode array detector (HPLC-DAD) method was employed for the quantification of chlorogenic acid, which resulted in a content of 10.05 mg/g crude extract. The results indicate the potential of Calystegia silvatica as a source of chlorogenic acid with cytotoxic activity.