Research Information System
The 9th International Mediterranean Symposium on Medicinal and Aromatic Plants, 3 - 05 May 2023, pp.60
Trachystemon orientalis (L.) G. Don, known as "kaldirik, hodan, ıspıt" in Türkiye, is an edible
plant that grows in Eastern Bulgaria, Western Caucasus, and Black Sea Region (1). It is used as a
food and folk medicine among the people (2,3). This study aimed to perform phytochemical and
antioxidant activity studies on the extracts prepared from the underground parts of the plant. The
isolation studies were carried out on the chloroform, ethyl acetate, and remaining aqueous
subextracts prepared from the methanol extract from the underground parts of the plant. The
structures of the compounds isolated were identified by 1H NMR and 13C NMR techniques.
FRAP and CUPRAC assays were performed to determine the antioxidant activity on the
methanol extract and the chloroform, ethyl acetate, and remaining aqueous fractions prepared
from the methanol extract. One phenolic acid (rosmarinic acid) was isolated from the ethyl
acetate fraction and one phenolic compound (3-(3,4-dihydroxyphenyl)-2-hydroxypropanoic acid
(danshensu)) and two arylnaphthalene lignans (globoidnan B and rabdosiin) from the remaining
aqueous fraction. These compounds were isolated from the underground parts of T. orientalis for
the first time. The ethyl acetate fraction showed the highest activity in the FRAP and CUPRAC
tests (794.818±8.999, 583.06±5.882 µM trolox equivalents (TE)/g), respectively. The rosmarinic
acid may be responsible for this activity.