Phytochemical and Antioxidant Activity Studies on the Underground Parts of Trachystemon orientalis


Koz A. N., Özgen U., Subaş T., Badem M., Kanbolat Ş., Demir A., ...More

The 9th International Mediterranean Symposium on Medicinal and Aromatic Plants, 3 - 05 May 2023, pp.60

  • Publication Type: Conference Paper / Summary Text
  • Page Numbers: pp.60
  • Karadeniz Technical University Affiliated: Yes

Abstract

Trachystemon orientalis (L.) G. Don, known as "kaldirik, hodan, ıspıt" in Türkiye, is an edible plant that grows in Eastern Bulgaria, Western Caucasus, and Black Sea Region (1). It is used as a food and folk medicine among the people (2,3). This study aimed to perform phytochemical and antioxidant activity studies on the extracts prepared from the underground parts of the plant. The isolation studies were carried out on the chloroform, ethyl acetate, and remaining aqueous subextracts prepared from the methanol extract from the underground parts of the plant. The structures of the compounds isolated were identified by 1H NMR and 13C NMR techniques. FRAP and CUPRAC assays were performed to determine the antioxidant activity on the methanol extract and the chloroform, ethyl acetate, and remaining aqueous fractions prepared from the methanol extract. One phenolic acid (rosmarinic acid) was isolated from the ethyl acetate fraction and one phenolic compound (3-(3,4-dihydroxyphenyl)-2-hydroxypropanoic acid (danshensu)) and two arylnaphthalene lignans (globoidnan B and rabdosiin) from the remaining aqueous fraction. These compounds were isolated from the underground parts of T. orientalis for the first time. The ethyl acetate fraction showed the highest activity in the FRAP and CUPRAC tests (794.818±8.999, 583.06±5.882 µM trolox equivalents (TE)/g), respectively. The rosmarinic acid may be responsible for this activity.