The concentration of eight different elements in tobacco of different region in Akcaabat (Trabzon/Turkey) was determined using energy dispersive X-ray fluorescence method. A radioisotope excited X-ray fluorescence analysis using the method of multiple standard addition is applied for the elemental analysis of tobacco and its ash. An annular 50 mCi(241)Am radioactive source and an annular 50 mCi(55)Fe radioactive source were used for excitation of characteristic K X-rays. An Si(Li) detector which has a 147 eV full width at. half maximum for 5.9 keV photons was used for intensity measurements. A qualitative analysis of spectral peaks showed that the samples contained potassium, calcium, titanium, iron, copper, bromine, strontium and barium. Evaluation of these elements with their potential hazards for smokers is briefly discussed. (C)2003 Published by Elsevier Science Ltd.