This paper presents the results of karyological analysis of seven Alchemilla species collected from north-east Anatolia, Turkey, belonging to Alchemilla sect. Alchemilla subsect. Heliodrosium ser. Vulgares and subsect. Calycanthurn ser. Elatae and ser. Calycinae. The following chromosome numbers were determined: A. haraldi 2n = 85-105, A. heterophylla 2n = 85-97, A. hirtipedicellata 2n = 86-100, A. oriturcica 2n = 86-102, A. persica 2n = 78-99, A. procerrima 2n = 69-78 and A. trabzonica 2n = 78-88. The chromosome numbers of three of these seven species are presented for the first time.