Liver biopsy is gold standard for diagnosis of liver inflammation, necrosis and fibrosis. In this study; we aimed to analyze the clinical indications of percutaneous liver biopsy, evaluation of histopathological findings, and biopsy related complications in children. Material and Methods: We retrospectively analyzed 75 children (50 male, 7.0±5.9 age, 2 months-16 years) who performed blind liver biopsy in Karadeniz Technical University Medical Faculty Pediatric Gastroenterology, Hepatology and Nutrition Department between January 2008 and March 2013. Age, gender, biopsy indications, histopathological findings, biopsy related complications and therapeutical approach were recorded from the hospital files. Results: Biopsies were performed to 19 patients with a diagnosis of chronic hepatitis B for pre-treatment evaluation. Biopsy indications were neonatal cholestasis in 9 patients (12%), Wilson disease in 9 (12%), isolated transaminase elevation in 13 (17.3%), storage disease in 9 (12%), autoimmune hepatitis in 4 (5.3%), metabolic liver disease in 9 (12%), acute hepatitis in 1 (1.3%) and hepatocelluler carcinoma in 1 patient (1.3%). Apart from the patients with chronic viral hepatitis B; 42 of the 56 patients (75%) had the final diagnosis with the assistance of histopathological findings of biopsy specimen. Biopsy specimen was insufficient in 1 patient (1.3%); while histopathological examination was normal in 2 patients (2.6%). Two patients had major complications after the biopsy (2.6%). One had hemobilia, and the other patient had intrahepatic hematoma and abdominal bleeding developed after biopsy. Conclusion: Liver biopsy is still the best method of determining liver disease. Biopsy related complications are rare in children. Althought the complications are rare, it must be performed after analyzing the risks and benefits. © 2014 by Türkiye Klinikleri.