Akademik Veri Yönetim Sistemi
Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium, Washington, Amerika Birleşik Devletleri, 9 - 13 Nisan 2019, cilt.40, ss.664, (Tam Metin Bildiri)
Mammalian cell behavior is guided by a microenvironment that provides chemical, physical, and biological cues through interactions between the extracellular matrix (ECM), other cells, and soluble factors. Demystifying this relationship between cell behavior and the environments they occupy remains an important goal across biomedical disciplines. A major challenge lies in recapitulating the complexity of the microenvironment in a systematic way to understand and direct cell fate. Here, we leverage recent advances in developing 3D engineered extracellular matrices (eECMs) to systematically profile the effects of ECM composition in driving cell behavior using high-resolution single-cell transcriptomics profiling and compare these behaviors to responses observed on 2D and other 3D substrates. We specifically target the influence of these environments in driving embryonic stem cell behavior and altering breast cancer stem cell populations.