Akademik Veri Yönetim Sistemi
V. International Enzyme and Bioprocess Days EBDays 2024 , İzmir, Türkiye, 27 - 29 Ağustos 2024, ss.98
Enzymes are complex organic molecules found in living cells. Starch is an important carbohydrate
stored in plant cells as a storage material for organisms. Starch is hydrolysed into glucose, maltose, and
oligosaccharides by enzymes such as α-, β-amylase, and pullulanase.In this study, 18 mesophilic and
thermophilic bacterial isolates, isolated and characterized in the Molecular Biology research laboratory
of the Department of Biology, Faculty of Science at KTU, were screened to produce α-amylase, βamylase, and pullulanase. For this purpose, hydrolysis tests were first carried out on solid media
containing starch and pullulan. Then, the activities of amylase and pullulanase enzymes showing
activity in solid media were determined by the DNS method from the isolated bacteria, and two strains
with the potential to produce all three enzymes were prioritized. Enzyme characterization studies were
carried out on the two strains showing the best enzyme activity, "Bacillus subtilis (Kont) and Bacillus
subtilis (KA4)", The reaction products of the amylase enzymes isolated from Bacillus subtilis (Kont)
and Bacillus subtilis (KA4) strains were analyzed using TLC. Then, the starch solution prepared for
the study on sweetening the corn starch (glucose syrup production) was treated with enzymes. The
reducing sugar determination and glucose content of the samples were determined by the
spectrophotometric method using DNS. It was determined that the amount of glucose initially present
in the medium increased after enzyme treatment. The reaction products were determined by
separations using TLC, and then the reaction products were analyzed by HPLC to determine the starch
hydrolysis products