Akademik Veri Yönetim Sistemi
I. International Apitherapy and Nature Congress, Nakhchivan, Azerbaycan, 1 - 03 Haziran 2023, ss.1
Propolis (bee gum) is the general
name of the resinous substance that honey bees collect from plant buds and
sprouts. The name propolis means defense in Greek and is responsible for the
protection of bees and the hive. Propolis shows significant bioactive
properties (antioxidant, antiviral, antifungal and antiparasitic activities).
The pharmacological effect of propolis against many viral infections has been
revealed.
In this study, it was determined that the aqueous propolis extract
inhibited the HIV reverse transcriptase activity, which plays a key role in the
replication mechanism of the HIV and is responsible for the conversion of single-stranded
virus RNA into double-stranded DNA. The aqueous extracts of propolis samples
were taken from Trabzon. The extracts were first passed through 2 kDa, 10 kDa and 30
kDa concentrators. Less than 2 kDa filtrates fractioned by HPLC. It was investigated that there are 18 phenolic compounds in 2 kDA fractions
of the propolis samples and this identified phenolic compound were tested for
the inhibition assays of HIV-1 RT enzyme by ELISA method. IC50
values of 18 phenolic compounds were determined with this method. The IC50
values of compounds Quercetin, Fisetin, Tannic acid, Pinocembrin, Mrycetin,
were 0.41; 0.92; 0.58, 0.023 and 0.09 mM. The inhibition properties of 6
phenolic compounds with IC50 value less than 1 mM, binary and 6-fold
combinations with IC50 value were measured, it was determined that
most of the combinations showed a synergistic effect, especially the 6-way
combination was equivalent to Nevirapine used in the treatment of AIDS today.
Keywords: ELISA, HIV-RT, inhibition, propolis