An investigation of the effects of chronic zonisamide, sultiam, lacosamide, clobazam, and rufinamide anti-seizure medications on foliculogenesis in ovarian tissue in prepubertal non-epileptic rats

Kart P. Ö., Gürgen S. G., ESENÜLKÜ G., Dilber B., YILDIZ N., YAZAR U., ...Daha Fazla

International Journal of Developmental Neuroscience, cilt.82, sa.5, ss.436-446, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 82 Sayı: 5
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1002/jdn.10200
  • Dergi Adı: International Journal of Developmental Neuroscience
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, EMBASE, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.436-446
  • Anahtar Kelimeler: anti-seizures medication, apoptosis, EGF, epilepsy, GDF-9, IGF-1, ovary, VALPROIC ACID, ANTIEPILEPTIC DRUGS, GROWTH, WOMEN, OXCARBAZEPINE, EPILEPSY, CARBAMAZEPINE, EXPRESSION, FEMALE
  • Karadeniz Teknik Üniversitesi Adresli: Evet

Özet

© 2022 International Society for Developmental Neuroscience.We aimed to determine the morphological and histological effects of zonisamide, sultiam, lacosamide, clobazam, and rufinamide on ovarian folliculogenesis in rats. Sixty female Wistar rats were divided into six experimental groups as control, zonisamide, sultiam, lacosamide, clobazam, and rufinamide groups; control solution and drugs were administered by gavage for 90 days. The number of healthy follicles in the control group was significantly higher than in the anti-medication groups (p < 0.001), and the number of corpus luteum was significantly lower (p < 0.001). There was a significant difference in the number of TUNEL positive apoptotic follicles between the control and drug groups (p < 0.001). With EGF, IGF-1, and GDF-9 staining, a very strong immunoreaction was observed in the ovarian multilaminar primary follicle granulosa cells and oocytes in the control group compared to the drug group (p < 0.001). Long-term anti-seizure medication with zonisamide, sultiam, lacosamide, clobazam, and rufinamide from prepubertal to adulthood causes apoptosis and disruption of folliculogenesis in the ovarian follicles of nonepileptic rats.