Akademik Veri Yönetim Sistemi
Jinekoloji ve Obstetrik Dergisi, cilt.18, sa.2, ss.101-104, 2004 (Scopus)
OBJECTIVE: The analysis of leukocytes in human semen could be useful in diagnosing male genital infections and infertility. Routine seminal leukocyte analysis is carried out by direct microscopic examination and peroxidase test. Recently, flow cytometry has been started to use for semen analysis in andrology laboratories. The purpose of this study was to compare flow cytometry to direct microscopic examination and the peroxidase test for seminal leukocyte analysis. STUDY DESIGN: Samples of ejaculated semen from 45 male patients were analyzed. Direct microscopy, peroxidase test and flow cytometric analysis were performed for seminal leukocyte analysis. Sensitivity and specificity of direct microscopic methods and peroxidase test for polymorphonuclear leukocytes were studied considering flow cytometry as the reference technique. RESULT(s): When flow cytometry is taken as reference technique, direct light microscopy had 60.6% sensitivity and 83.3% specificity, and peroxidase test had 81.8% sensitivity and 91.6% specificity in evaluating seminal samples for leukocytes. CONCLUSION(s): We have concluded that flow cytometric analysis using monoclonal antibodies is a better objective method than direct microscopy and peroxidase test, enabling seminal leukocytes to be accurately detected.