Akademik Veri Yönetim Sistemi
Fisheries and Aquatic Sciences, cilt.29, sa.1, ss.31-43, 2026 (Scopus)
Ovarian lavage sperm artificial insemination (OLSAI) is a human-assisted fish breeding technique that involves delivering sperm into the ovary via a catheter tube, which allows internal gamete association. This systematic review and meta-analysis compared OLSAI to dry external fertilization procedures, with the parameters focused on fertilization and hatching rates. We conducted a literature search on only external fertilization fish species to collect publications. After screening and satisfying the inclusion criteria, the final systematic review and meta-analysis included eleven peer-reviewed articles.We conducted random-effects meta-analyses to incorporate data on fertilization and hatching rates, given the anticipated heterogeneity. The trim and fill analysis revealed significant variability between studies. The fertilization and hatching rate Tau-squared (τ2) values were 910.57 and 1313.98, respectively. Moreover, heterogeneity statistics indicated a higher I2, representing a 97.05% fertilization rate and a 99.12% hatching rate. The pooled rates of fertilization for OLSAI and dry external fertilization were 52.78% and 67.92%, respectively. The pooled hatching rate was also 52.91% for OLSAI and 73.89% for dry external fertilization. De-spite these numerical differences, aWelch two-sample t-test revealed no significant difference between OLSAI and dry external fertilization in either fertilization or hatching rates, likely due to several sources of heterogeneity. This study revealed that OLSAI could be an alternative method of freshwater fish species propagation to the conventional dry external fertilization technique without compromising the success rate. To capitalize on the benefits of OLSAI and contribute to advancing our knowledge, further research across species is needed.