Comparison of pulsed-field gel electrophoresis and enterobacterial repetitive intergenic consensus PCR and biochemical tests to characterize Lactococcus garvieae


Ture M., Altinok I. , Capkin E.

JOURNAL OF FISH DISEASES, cilt.38, ss.37-47, 2015 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 38 Konu: 1
  • Basım Tarihi: 2015
  • Doi Numarası: 10.1111/jfd.12192
  • Dergi Adı: JOURNAL OF FISH DISEASES
  • Sayfa Sayıları: ss.37-47

Özet

Biochemical test, pulsed-field gel electrophoresis (PFGE) and enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR) were used to compare 42 strains of Lactococcus garvieae isolated from different regions of Turkey, Italy, France and Spain. Twenty biotypes of L.garvieae were formed based on 54 biochemical tests. ERIC-PCR of genomic DNA from different L.garvieae strains resulted in amplification of multiple fragments of DNA in sizes ranging between 200 and 5000bp with various band intensities. After cutting DNA with ApaI restriction enzyme and running on the PFGE, 11-22 resolvable bands ranging from 2 to 194kb were observed. Turkish isolates were grouped into two clusters, and only A58 (Italy) strain was connected with Turkish isolates. Similarities between Turkish, Spanish, Italian and French isolates were <50% except 216-6 Rize strain. In Turkey, first lactococcosis occurred in Mugla, and then, it has been spread all over the country. Based on ERIC-PCR, Spanish and Italian strains of L.garvieae were related to Mugla strains. Therefore, after comparing PFGE profiles, ERIC-PCR profiles and phenotypic characteristics of 42 strains of L.garvieae, there were no relationships found between these three typing methods. PFGE method was more discriminative than the other methods.