This paper presents the results of karyological analysis of four species from the section Alchemilla ROTHM., subsection Calycantum ROTHM., series Calycinae Bus. Specimens studied karyologically originated from Northeast Anatolia (Turkey). Their chromosome numbers were as follows: A. transcaucasica ROTHM. 2n = 128 - 140; A. retinervis Bus. 2n = 101 - 106; A. ellenbergiana ROTHM. 2n = 120 - 132; A. dura Bus. 2n = 108 - 126. From cytological point of view Alchemilla represents a difficult object. All studied species from Northeast Anatolia are proven to be high polyploids like species of Alchemilla investigated from various geographic areas.