Akademik Veri Yönetim Sistemi
Progress in Nutrition, cilt.24, sa.1, 2022 (SCI-Expanded)
© Mattioli 1885Ethanolic propolis extract (EPE) can be used as a food supplement since it is a rich source of phenolic compounds. However, it is also suspected that a high level of phenolic substances may interact with blood-thinning drugs, such as warfarin. This preliminary study investigated the effect of warfarin combined with EPE on blood coagulation in rats. Prothrombin time (PT), international normalized ratio (INR), activated partial thromboplastin time (aPTT), and fibrinogen were measured in all experimental groups to determine blood coagulation effects. In addition, the propolis extract was analyzed as in vitro. The total amounts of phenolic and flavonoid substances were 82.40±0.44 mg of gallic acid equivalents (GAE)/mL and 28.62±0.18 mg of quercetin equivalents (QUE)/mL, respectively. According to the HPLC analysis, rutin, caffeic acid phenyl ester (CAPE), and chrysin were identified as major components. Moreover, while the EPE [200 mg/kg, body weight (bw)] did not significantly affect coagulation parameters when used alone, it caused mild changes in combination with warfarin. When compared to the control group (G1), the levels of PT and fibrinogen of the combination group (G4) observed significant statistical difference (p≤0.005). However, the increases in all blood coagulation factors except fibrinogen were found to be higher in the warfarin group (G2) and these differences were also statistically confirmed (p≤0.005). In conclusion, the data obtained may be interpreted as evidence of a synergistic effect between the applied dose of EPE and warfarin, despite the expectation of a possible drug interaction.