Bee Venom Induces the Interaction between Phosphorylated Histone Variant, H2AX, and the Intracellular Site of beta-Actin in Liver and Breast Cancer Cells

Tetikoğlu S., Çelik Uzuner S.

CHEMISTRY & BIODIVERSITY, cilt.20, sa.6, 2023 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 20 Sayı: 6
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1002/cbdv.202300401
  • Dergi Adı: CHEMISTRY & BIODIVERSITY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Agricultural & Environmental Science Database, Aquatic Science & Fisheries Abstracts (ASFA), CAB Abstracts, Chemical Abstracts Core, EMBASE, MEDLINE, Veterinary Science Database
  • Anahtar Kelimeler: actin, bee venom, cancer, DNA damage, H2AX
  • Karadeniz Teknik Üniversitesi Adresli: Evet

Özet

Bee venom is a natural mixture and candidate anti-cancer agent with selective cytotoxic effect on some cancer cells. However, the cellular mechanisms of how bee venom selectively targets cancer cells remain elusive. The aim of this study was to reveal the genotoxic effect of bee venom in concordance with the location of beta-actin protein throughout the nucleus or/and cytoplasm. For this aim, the level of H2AX phosphorylation (gamma H2AX) and intracellular location of beta-actin were assessed by immunofluorescence in liver (HEPG2) and metastatic breast (MDA-MB-231) cancer cell lines compared to normal fibroblasts (NIH3T3) after bee venom treatment. Colocalisation profiles of gamma H2AX and beta-actin in each cell line were also analysed. The results showed that the levels of gamma H2AX staining decreased in normal cells but increased in cancer cells. The majority of beta-actin was localised within the cytoplasm of normal cells after bee venom treatment, but it was mostly accumulated within the nucleus in cancer cells. Colocalisation of beta-actin and gamma H2AX both in nucleus and cytoplasm was induced in each cancer cell by different patterns. The results showed that normal and cancerous cells had different responses against bee venom, and suggested that bee venom induced a cellular response by the interaction between gamma H2AX and beta-actin.