Identification and pathogenicity of bacteria from European shot-hole borer, Xyleborus dispar Fabricius (Coleoptera : Scolytidae)

SEZEN K., Kati H., NALÇACIOĞLU R., MURATOĞLU H., DEMİRBAĞ Z.

ANNALS OF MICROBIOLOGY, cilt.58, sa.2, ss.173-179, 2008 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 58 Sayı: 2
  • Basım Tarihi: 2008
  • Doi Numarası: 10.1007/bf03175313
  • Dergi Adı: ANNALS OF MICROBIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.173-179
  • Anahtar Kelimeler: Bacillus thuringiensis, microbiota, microbial control, Xyleborus dispar, BIOLOGICAL-CONTROL AGENT, BACILLUS-THURINGIENSIS, ESCHERICHIA-COLI, CRYSTAL PROTEINS, DELTA-ENDOTOXIN, LEPIDOPTERA, STRAINS, L., TEPHRITIDAE, SEQUENCE
  • Karadeniz Teknik Üniversitesi Adresli: Evet

Özet

In this study, we have isolated and identified 5 bacterial isolates from European shot-hole borer, Xyleborus dispar (Coleoptera: Scolytidae), an important pest of hazelnut. After various morphological, physiological, biochemical and molecular characteristics were determined in detail, bacterial isolates were identified as Pseudomonas fluorescens (Xd1), Bacillus megaterium (Xd2), Bacillus thuringiensis (Xd3), Pseudomonas rhizosphaerae (Xd4) and Pantoea cedenensis (Xd5). Especially, the B. thuringiensis strain was identified in more detail as Bacillus thuringiensis subsp. tenebrionis (H8ab). The insecticidal activities were determined against the larvae of Agelastica alni L. (Col.: Chrysomelidae), Amphimallon solstitiale L. (Col.: Scarabaeidae), Leptinotarsa decemlineata (Say) (Col.: Chrysomelidae), and Melolontha melolontha L. (Col.: Scarabaeidae) larvae at 1.8 x 10(9) CFU/ml dose, within five days. The highest insecticidal activities are 100, 100, 80 and 100% for Bacillus thuringiensis subsp. tenebrionis (Xd3) on A. alni, A. solstitiale, L. decemlineata, and M. melolontha larvae, respectively. Our results indicate that especially B. thuringiensis subsp. tenebrionis (Xd3) may be valuable as a microbial control agent for coleopteran pests, respectively.