Selection of mimotopes of Bovine Viral Diarrhoea Virus using a solid-phase peptide library

Kalaycioglu A. T., Russell P. H., Howard C. R.

VACCINE, vol.25, no.41, pp.7081-7086, 2007 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 25 Issue: 41
  • Publication Date: 2007
  • Doi Number: 10.1016/j.vaccine.2007.07.066
  • Journal Name: VACCINE
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.7081-7086
  • Keywords: bovine viral diarrhoea virus, BVDV, mimotope, marker vaccine, RESPIRATORY SYNCYTIAL VIRUS, MONOCLONAL-ANTIBODIES, NEUTRALIZING ANTIBODIES, MUCOSAL DISEASE, EPITOPE, BINDING, PROTEIN, CATTLE, BVDV, E2
  • Karadeniz Technical University Affiliated: No

Abstract

Bovine Viral Diarrhoea Virus (BVDV) is an important pathogen of cattle, causing important economical losses world-wide. In this study, an 8-mer solid-phase peptide library was screened with a neutralising monoclonal antibody 157 to generate mimotopes mimicking a conformational neutralising epitope of BVDV E2 protein. Two sequences selected 157A1 LFEQYYYF and 157A2 LYRFGEFD that did not show a high structural or sequence similarity with BVDV E2 glycoprotein reacted specifically with monoclonal antibody 157 when presented as solid-phase peptides in a SPOT scan assay. These results indicate that combinatorial peptide libraries can be used to identify potential mimotopes of conformational epitopes. (c) 2007 Elsevier Ltd. All rights reserved.