Development of Beauveria bassiana (Ascomycota: Hypocreales) as a mycoinsecticide to control green peach aphid, Myzus persicae (Homoptera: Aphididae) and investigation of its biocontrol potential

Biryol S., DEMİRBAĞ Z., Erdogan P., DEMİR İ.

JOURNAL OF ASIA-PACIFIC ENTOMOLOGY, cilt.25, sa.1, 2022 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 25 Sayı: 1
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1016/j.aspen.2022.101878
  • Dergi Adı: JOURNAL OF ASIA-PACIFIC ENTOMOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Veterinary Science Database
  • Anahtar Kelimeler: Beauveria bassiana, Liquid-state fermentation, Myzus persicae, Oil-based mycoinsecticide, DESICCATION TOLERANT BLASTOSPORES, FUNGUS METARHIZIUM-ANISOPLIAE, LADY BEETLE COLEOPTERA, OIL-BASED FORMULATION, ENTOMOPATHOGENIC FUNGI, BIOLOGICAL-CONTROL, ISARIA-FUMOSOROSEA, LECANICILLIUM-ATTENUATUM, CONIDIAL GERMINATION, HEMIPTERA APHIDIDAE
  • Karadeniz Teknik Üniversitesi Adresli: Evet

Özet

Long-term and intensive use of synthetic insecticides to control the green peach aphid, Myzus persicae (Homoptera: Aphididae) in agricultural production in the world has resulted in pest resistance and environmental pollution. The aim of the study was to develop an environmentally-friendly and effective mycoinsecticide from a local fungal isolate against M. persicae. According to the results of the screening experiments using 15 isolates (6 x Metarhizium, 5 x Beauveria, 2 x Isaria, 2 x Lecanicillium) at 1 x 10(7) conidia ml(-1) concentrations and the dose-response experiments at 1 x 10(5) -1 x 10(9) conidia ml(-1) concentrations against M. persicae nymphs, Beauveria bassiana (KTU-24) was identified as the most promising isolate. The strain KTU-24 is also had tolerance to ecological conditions such as temperature, and UV-B. KTU-24 with advantageous and superior properties was used to develop a test mycoinsecticide. Mass production of spores by KTU-24 was conducted out by liquid-state fermentation using liquid medium. Spores harvested from the sporulated biomass were used to develop an oil-based mycoinsecticide, and the product was designated as AFIDISIDAL-OD Bbas-TR61. The product had lethal effect on M. persicae nymphs at a concentration of 1 x 10(8) conidia ml(-1) in leaf-disc (82.5%) and pot (84.33%) experiments in a climate chamber. The oil-based mycoinsecticide developed in this study could be profitable when used in aphid-IPM prgrams by reducing crop loss and synthetic pesticides use.