Comparative effects of metformin and Cistus laurifolius L. extract in streptozotocin-induced diabetic rat model: oxidative, inflammatory, apoptotic, and histopathological analyzes

Hacioglu C., KAR F., KARA Y., Yucel E., Donmez D. B., ŞENTÜRK H., ...More

ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, vol.28, no.41, pp.57888-57901, 2021 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 28 Issue: 41
  • Publication Date: 2021
  • Doi Number: 10.1007/s11356-021-14780-y
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, IBZ Online, ABI/INFORM, Aerospace Database, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, EMBASE, Environment Index, Geobase, MEDLINE, Pollution Abstracts, Veterinary Science Database, Civil Engineering Abstracts
  • Page Numbers: pp.57888-57901
  • Keywords: Diabetes, Cistus laurifolius L, Metformin, Oxidative stress, Apoptosis, Inflammation, Histopathology, PHENOLIC-COMPOUNDS, IN-VIVO, FLAVONOIDS, PATHOPHYSIOLOGY, ACTIVATION, FRACTIONS, MECHANISM, GLUCOSE, LEAVES, CELLS
  • Karadeniz Technical University Affiliated: Yes


Interest in phytochemical therapy methods in the treatment of diabetes is increasing day by day. Although the antidiabetic and antioxidant effects of Cistus laurifolius L. (CL) have been mentioned, the systemic effects remain unknown. The present study aims at evaluating the antidiabetic effects of the CL aqueous extract via metformin on streptozotocin (STZ)-induced diabetic rats. Forty male Wistar albino rats were divided into five groups of eight animals each: control, diabetic group (55mg/kg STZ), STZ+125mg/kg CL, STZ+250mg/kg CL, and STZ+100mg/kg metformin. The effects of CL and metformin on oxidative, apoptotic, and inflammatory pathways were comparatively investigated. In addition, nuclear factor-kappa B (NF kappa B), tumor necrosis factor-alpha (TNF-alpha), and interleukin (IL)-1 beta expressions analysis were carried out. CL treatment resulted in a significant improvement in blood glucose levels, lipid profile, pancreatic markers, and liver and kidney function tests. A 250mg/kg CL treatment decreased by 67.9%, 31.6%, 66.8%, 28.3%, and 31.4% in the total oxidant capacity, NF kappa B, TNF-alpha, IL-1 beta, caspase3, and cytochrome c levels, respectively, compared to the diabetic group. Additionally, CL treatments showed a dose-dependent reduction in NF kappa B, TNF-alpha, and IL-1 beta expression levels. A 250mg/kg CL treatment exhibited a greater increase (by 9.6%) in total antioxidant capacity than metformin. CL treatment provided histologically more improvement in the brain, heart, pancreas, spleen, liver, kidney, and testicular tissues compared to the metformin group. Our results suggest that the single treatment of CL aqueous extract at the low doses may have stronger short-term anti-diabetic effects than metformin. Therefore, further studies are needed regarding the long-term hypoglycemic effect or treatment of CL aqueous extract.