In the present study, a macrofungus, Hypholoma fasciculare (H. fasciculare), was employed for the biosorption of vanadium from aqueous solutions. The characterization of H. fasciatlare was conducted using the FT-IR and SEM-EDS techniques before and after biosorption. The batch experimental parameters that affect the biosorption of vanadium on the macrofungus were optimized. The Langmuir, Freundlich, and D-R isotherms were employed, and it was found that the Langmuir and D-R models best explained the adsorption mechanism. The kinetic and thermodynamic characteristics of the biosorption mechanism were investigated in detail. The Langmuir biosorption capacity was found as 159.7 mg/g. The thermodynamic parameters showed that the biosorption of vanadium on the macrofungus was more feasible by decreasing the temperature and the exothermic character of Delta G degrees= -20.6 kJ/mol at 293 K and Delta G degrees= -19.9 kJ/mol at 323 K; Delta H degrees= -26.9 kJ/mol, Delta S degrees= -21.8 J/mol. K. The biosorption kinetics of vanadium onto the macrofungus H. lascictifare was best fitted with pseudo-second-order (R-2 >= 0.99). The desorption studies showed that 0.50 M of HNO3 effectively desorbs vanadium from H. fasciculare.