Molecular characterization of the Vairimorpha (Nosema) ceranae infection from Bombus terrestris (Linnaeus, 1758) (Hymenoptera: Apidae) in Turkey

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TOSUN O., Beki̇Rcan Ç.

Journal of the Entomological Research Society, vol.23, no.3, 2021 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 23 Issue: 3
  • Publication Date: 2021
  • Doi Number: 10.51963/jers.v23i3.2101
  • Journal Name: Journal of the Entomological Research Society
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Veterinary Science Database
  • Keywords: Bombus terrestris, microsporidiosis, RPB1, SSU rRNA, Vairimorpha ceranae, HONEY-BEE, APIS-MELLIFERA, MICROSPORIDIAN PARASITE, DECLINE, BUMBLEBEES, CONSERVATION, PATHOGEN, SEQUENCE, SPP.
  • Karadeniz Technical University Affiliated: Yes

Abstract

© 2021 Gazi Entomological Research Society. All rights reserved.The current study aimed to determine the natural Microsporidium pathogen of the Bombus terrestris L. (Hymenoptera: Apidae) in Turkey (Mersin, Antalya, Muğla, İzmir, Aydın). During 2019 and 2020, the commercial and wild populations of B. terrestris were investigated in this survey. In the studies, natural microsporidiosis was detected in commercial B. terrestris populations. Fresh oval spores were measured as 4.91 ± 0.48 (6.12 - 3.73) µm in length and 2.54 ± 0.31 (3.27 - 1.88) µm in width (n=60). Both SSU rRNA and RPB1 gene sequences of the current microsporidium were top hits with the Vairimorpha (Nosema) ceranae isolates. While the SSU rRNA gene sequence matched with the Vairimorpha ceranae clone NCS44 (LC510228) isolated from the Apis cerana japonica at 99.24% identity (100% coverage), the RPB1 gene sequence was matched with the Vairimorpha ceranae isolate 1994 (KJ473287) at 99.02% identity (100% coverage). Based on the light microscopy and molecular phylogeny the current microsporidium was a new isolate of the V. ceranae and named here in as Vairimorpha ceranae Tr-07.