Identification of AMV063 as a Caspase-2 Protein in Amsacta moorei entomopoxvirus
International Congress on Invertebrate Pathology and Microbial Control, Den Bosch, Hollanda, 2 - 06 Ağustos 2026, ss.91-92, (Özet Bildiri)
- Yayın Türü: Bildiri / Özet Bildiri
- Basıldığı Şehir: Den Bosch
- Basıldığı Ülke: Hollanda
- Sayfa Sayıları: ss.91-92
- Karadeniz Teknik Üniversitesi Adresli: Evet
Özet
Amsacta moorei entomopoxvirus (AMEV), a member of the Poxviridae family, can be cultivated and genetically manipulated in insect cell lines. In insect viruses, apoptosis is regulated by molecular mediators, including caspase activation and the expression of inhibitor of apoptosis proteins, which are central to virus–host interactions. Caspases play critical roles in viral life cycles, contributing to replication, maturation, and dissemination by releasing progeny virions from infected cells. Previous in silico analyses have indicated that the AMV063 protein of AMEV may BE a putative Caspase-2 protein. This study was designed to elucidate the functional caspase role of the AMV063 protein in viral infectivity and apoptosis within the host. To this aim, a recombinant AMEV (AmΔ063/gfp) had been constructed previously. In this work, the AMV063 protein was expressed recombinantly using the Bac-to-Bac baculovirus expression system and subsequently purified. Apoptosis-sensitive cells were then treated with various combinations of the recombinant virus (AmΔ063/gfp), a control virus (AmΔsph/gfp), and the purified AMV063 protein. Caspase-2 and TUNEL activity of the AMV063 ORF were investigated by comparing them with recombinant and wild-type viruses.
This project was funded by KTÜ-BAP (FYL-2018-7782 and FBA-2024-15913)