l-Arginine Improves Sperm Motility in Threatened Coruh Trout Salmo coruhensis (Teleostei: Salmonidae)

KOCABAŞ M., Kutluyer F., Erisir M., Benzer F.

IRANIAN JOURNAL OF SCIENCE AND TECHNOLOGY TRANSACTION A-SCIENCE, vol.43, pp.1433-1437, 2019 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 43
  • Publication Date: 2019
  • Doi Number: 10.1007/s40995-018-0648-z
  • Journal Name: IRANIAN JOURNAL OF SCIENCE AND TECHNOLOGY TRANSACTION A-SCIENCE
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1433-1437
  • Keywords: Amino acid, Spermatozoa, Trout, Nitric oxide, NITRIC-OXIDE, RAINBOW-TROUT, QUALITY, SPERMATOZOA, CRYOPRESERVATION, FERTILIZATION, SEMEN, MILT
  • Karadeniz Technical University Affiliated: Yes

Abstract

l-arginine is a versatile amino acid and precursor of nitric oxide that provides protection for preventing free radical damage. Herein, experiments were designed to clarify the effect of l-arginine supplementation on the sperm motility of a threatened trout, Coruh trout Salmo coruhensis. Activation solution was supplemented with levels of 0mM (Control), 2mM, 4mM, and 8mMl-arginine, and motility and survival of sperm cells were assessed. The selected concentrations were determined according to results of our preliminary experiments. The sperm motility rates at l-arginine concentrations of 0, 2, 4, and 8mM were 73.33 +/- 5.77%, 76.67 +/- 5.75%, 80.01 +/- 0.01%, and 90.00 +/- 0.05%, respectively; notility durations were 21.67 +/- 2.08, 32.33 +/- 3.79, 32.67 +/- 1.15, and 37.00 +/- 6.38s, respectively. Significant effect of l-arginine supplementation was determined on the percentage and duration of motile spermatozoa (p<0.05). The maximum increment was evoked at the greatest concentration (8mM). Here, we showed that l-arginine supplement can improve sperm motility of S. coruhensis.