Akademik Veri Yönetim Sistemi
26. Ulusal ve 1. Uluslararası Farmakoloji Kongresi, 4 - 06 Kasım 2021, ss.455-456
Objectıve: Senecio trapezuntinus Boiss (Asteracea), an endemic species in Turkey, is known as 'Trabzon canary grass'. Senecio species are
used for amenorrhea, dysmenorrhea and infertility in folk medicine. Senecio coronatus has been known to induce labor. The effect of Senecio
trapezuntinus on uterine contractility is unknown. In this study, the effects of hexane extract prepared from the aerial parts of (STHE) on
mouse isolated uterine smooth muscle contraction were investigated.
Materials-Methods: The aerial parts of Senecio trapezuntinus Boiss were dried and ground. In order to prepare STHE, the powder sample
was extracted with methanol for 8 hours at 40°C, it was concentrated in a rotary evaporator. STHE was obtained from the methanolic extract
with n-hexane in a separatory funnel. The effects of STHE on uterine smooth muscle contraction was evaluated in vitro by isolated organ
bath method. 10-12 weeks old non-pregnant female Balb/c mice (n=3-4) were anesthetised with ketamine-xylazine and the uterine horns
were isolated. Two uterine ring preparations (2-3 cm length) were obtained from each mouse. Uterine rings were mounted longitudinally in
organ baths containing Krebs-Henseleit solution gassed with a mixture of 95% O2 + 5% CO2 at 37 °C under 1 g of resting tension and isometric
contractions of uterine rings were recorded. Then, the concentration-dependent relaxation response of STHE (0.01-0.25 mg/mL) were
obtained from uterine rings pre-contracted with oxytocin (0.1 IU/mL). Data were presented as percentage of area under the curve of
oxytocin-induced precontraction and statistically analyzed by Student-t test. P<0.05 was considered statistically significant (GraphPad Prism
5.0, USA).
Results: STHE (0.01-0.25 mg/mL) induced concentration-dependent relaxation response of uterine smooth muscle pre-contracted with
oxytocin (Emax=96.43±1.67%; EC50=0.25±0.10 mg/mL) and significantly reduced spontaneous smooth muscle contractions compared to control
(Emax=48.94±6.37%; p<0.05).
Conclusıon: Senecio trapezuntinus Boiss has therapeutic potential as a tocolytic. Further studies are conducted to evaluate the underlying
mechanisms.
*This study was supported by Ministry of Agriculture and Forestry, General Directorate of Nature Conservation and National Parks.
Keywords: Oxytocin, Senecio trapezuntinus Boiss, tocolytic