Dexmedetomidine acts as an oxidative damage prophylactic in rats exposed to ionizing radiation


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Kutanis D., Ertürk E., Beşir A., Demirci Y., Kayır S., Akdoğan A., ...Daha Fazla

JOURNAL OF CLINICAL ANESTHESIA, cilt.34, ss.577-585, 2016 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 34
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1016/j.jclinane.2016.06.031
  • Dergi Adı: JOURNAL OF CLINICAL ANESTHESIA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.577-585
  • Anahtar Kelimeler: Ionizing radiation, Oxidative damage, Dexmedetomidine, ISCHEMIA-REPERFUSION INJURY, PROPOFOL, PNEUMOPERITONEUM, MANAGEMENT, MELATONIN, RADICALS, PROTECTS, STRESS, LIVER
  • Karadeniz Teknik Üniversitesi Adresli: Evet

Özet

Study objective: To investigate the effects of dexmedetomidine on oxidative injury caused by ionizing radiation.
Design: Randomized controlled experimental study.
Setting: Department of radiation oncology and research laboratory of an academic hospital.
Interventions: Twenty-eight rats were randomized to 4 groups (n = 7 per group). Group S rats were administered physiologic serum; group SR rats were administered physiologic serum and 10 Gy external ionizing radiation. Groups D100 and D200 were administered 100 and 200 μg/kg dexmedetomidine intraperitoneally, respectively, 45 minutes before ionizing radiation.
Measurements: Liver, kidney, lung, and thyroid tissue and serum levels of antioxidant enzymes (glutathione peroxidase [GPX], superoxide dismutase, and catalase) and oxidative metabolites (advanced oxidation protein products, malondialdehyde, and nitrate/nitrite, and serum ischemia-modified albumin) were measured 6 hours postprocedure.
Main results: In group SR, IR decreased antioxidant enzyme levels and increased oxidative metabolite levels (P b .05). In plasma, antioxidant enzyme levels were higher and oxidative metabolite levels were lower in groups D100 and D200 than in group SR (P b .01). In tissues, hepatic and lung GPX levels were higher in groups D100 and D200 than in group SR (P b .001). Renal and thyroid GPX levels were higher in D200 than in group SR (P b .01). Thyroid superoxide dismutase levels were higher in groups D100 and D200 than in group SR (P b .01). Renal, lung, and thyroid catalase levels were higher in group D200 than in group SR (P b .01). Hepatic, renal, and lung advanced oxidation protein products and malondialdehyde levels were lower in groups D100 and D200 than in group SR (P b .01). Hepatic, renal, and lung nitrate/nitrite levels were lower in group D200 than in group SR (P b .05).
onclusions: Dexmedetomidine preserves the antioxidant enzyme levels and reduces toxic oxidant metabolites.
Therefore, it can provide protection from oxidative injury caused by ionizing radiation.