© 2020 Society of Pharmaceutical Sciences of Ankara (FABAD). All rights reserved.Origanum species are traditionally used frequently as spices and because of their medicinal effects. In this study, isolation studies were carried out by using column chromatography on Origanum micranthum Vogel and Origanum minutiflorum O. Schwarz & P.H. Davis. 4(3,4-dihydroxy benzoyl oxymethyl) phenyl-β-D-glucopyranoside (1), rosmarinic acid (2), 3-(3,4-di-hydroxyphenyl)-2-hydroxypropionic acid (3), ursolic acid (4A), oleanolic acid (4B) were isolated from O. micranthum, rosmarinic acid (5), apigenin (6) and vicenin-2 (7) were isolated from O. minutiflorum at the end of the study.