Akademik Veri Yönetim Sistemi
FEMS MICROBIOLOGY LETTERS, cilt.370, 2023 (SCI-Expanded)
Strain GK(T) was isolated from the Kumbet plateu of Giresun in Turkey. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain GK(T) belonged to genus Janthinobacterium and 16S rRNA gene sequence similarities with all type strains of the genus Janthinobacterium were 98.89%-99.78%. The calculated pairwise average nucleotide identity (ANI) values between strain GK(T) and all type strains of Janthinobacterium species were in the range of 79.8%-93.2%. In addition, digital DNA-DNA hybridization (dDDH) values were in the range of 23.0%-51.7%. Major fatty acids are C(10:0)3OH, C-12:0, C-16:1 omega 7c, C-16:0, and C-18:1 omega 7c, and polar lipids included phosphatidylethanolamine, phosphatidylglycerol, also one unidentified phospholipid and one unidentified aminophospholipid. The respiratory quinone of strain GK(T) was determinated to be Q-8. The genome sizes of strain GK(T) was 6 197 538 bp with 63.16% G + C ratio. Strain GK(T) is Gram-stain-negative, aerobic, rod-shaped, and motile. A violet pigment was produced by strain GK(T). The crude violacein pigments were separated into three diferent bands on a TLC sheet. Then violacein and deoxyviolacein were purifed by vacuum liquid column chromatography and identifed by NMR spectroscopy. The antimicrobial activities of purifed violacein and deoxyviolacein were screened for seven microorganisms. Based on the results of the morphological, biochemical, physiological, phylogenetic, and genomic characteristics, we propose classifying the strain GK(T) as representative of a novel species of the genus Janthinobacterium, for which the name Janthinobacterium kumbetense sp. nov. is proposed (GK(T) = LMG 32662(T) = DSM 11423(T)). Janthinobacterium kumbetense sp. nov., was isolated from spring water, and its violacein and deoxyviolacein were extracted and characterized and antimicrobial activities were determined.