PCR method for detection of largemouth bass virus

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Grizzle J., Altinok I., Noyes A.

DISEASES OF AQUATIC ORGANISMS, cilt.54, sa.1, ss.29-33, 2003 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 54 Sayı: 1
  • Basım Tarihi: 2003
  • Doi Numarası: 10.3354/dao054029
  • Dergi Adı: DISEASES OF AQUATIC ORGANISMS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.29-33
  • Anahtar Kelimeler: Micropterus salmoides, detection method, polymerase chain reaction, MOLECULAR CHARACTERIZATION, SEQUENCE-ANALYSIS, IRIDOVIRUS, FISH
  • Karadeniz Teknik Üniversitesi Adresli: Hayır

Özet

A polymerase chain reaction (PCR) method was developed for largemouth bass virus (LMBV). This iridovirus can cause a lethal disease of largemouth bass Micropterus salmoides, but also subclinically infects largemouth bass and other species of fishes. Oligonucleotide primers were designed to specifically amplify the major capsid protein gene of LMBV. The protocol for sample processing and PCR provided a method that was more sensitive than cell culture for detection of LMBV in fish. The specific amplification of LMBV also provided an improved method for confirming the identity of cell-culture isolates presumptively identified as LMBV.