The Effect of Chlorogenic Acid on 5-Fluorouracil-Induced Oxidative Damage in Rat Ovarian Tissue


Menteşe A., Demir S., Türkmen Alemdar N., Aliyazıcıoğlu Y., Değer O.

FARABİ TIP DERGİSİ, vol.1, no.1, pp.1-7, 2022 (Peer-Reviewed Journal)

  • Publication Type: Article / Article
  • Volume: 1 Issue: 1
  • Publication Date: 2022
  • Journal Name: FARABİ TIP DERGİSİ
  • Page Numbers: pp.1-7
  • Karadeniz Technical University Affiliated: Yes

Abstract

5-fluorouracil (5-FU) is a potent anticancer agent, but its significant tissue toxicity associated with increased oxidative stress and inflammation may limit its clinical use. Chlorogenic acid (CGA) is a dietary polyphenol found in a variety of foods and beverages, including coffee, apples, pears, strawberries, and grapes. It was aimed to evaluate the therapeutic effects of CGA on 5-FU-induced ovotoxicity through oxidative stress and inflammation parameters in this study. Thirty female Sprague-Dawley rats were divided into 5 groups with equal numbers of subjects (n=6): control, 5-FU (100 mg/kg), 5-FU+CGA (1.5 mg/kg), 5-FU+CGA (mg/kg) and CGA (3 mg/kg). The ovarian tissue levels of malondialdehyde (MDA), total oxidant status (TOS), total antioxidant status (TAS), 8-hydroxy-2' -deoxyguanosine (8-OHdG), catalase (CAT) and interleukin-6 (IL-6) were determined using commercial spectrophotometric kits. While 5-FU treatment increased MDA, TOS, 8-OHdG and IL-6 levels in ovarian tissue, it significantly decreased TAS and CAT levels (p<0.05). These parameters, indicating 5-FU-induced toxicity, were significantly reversed with CGA administrations in a dose-dependent manner. The results support the view that CGA may be a useful modulator in attenuating 5-FU-induced ovotoxicity.