Ploidy analysis and S-phase fraction determination by flow cytometry in anembryonic pregnancy and spontaneous abortions


Ozeren M., Aydemir V., Tekelioglu Y., Topcuoglu K., Bozkaya H.

GYNECOLOGIC AND OBSTETRIC INVESTIGATION, vol.48, no.2, pp.104-107, 1999 (Peer-Reviewed Journal) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 48 Issue: 2
  • Publication Date: 1999
  • Doi Number: 10.1159/000010149
  • Journal Name: GYNECOLOGIC AND OBSTETRIC INVESTIGATION
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.104-107

Abstract

The nuclear DNA content of 20 anembryonic pregnancies was studied by flow cytometry from paraffin embedded tissue blocks. An abnormal amount of DNA content was found in 8 of the cases. This was a significantly higher percentage than encountered in spontaneous abortions studied by the same population (40 and 9%, respectively, p < 0.05). The S-phase fraction in anembryonic pregnancies was lower than in spontaneous abortions (22.4 +/- 12.7 and 35.4 +/- 6.8, respectively, p < 0.01). The results indicated that abnormal embryogenesis with grave chromosomal aberrations may play a major role in the etiology of anembryonic pregnancy.