Effect of ethyl pyruvate on oxidative state and endoplasmic reticulum stress in a rat model of testicular torsion


Demir S., Kazaz İ. O. , Aliyazıcıoğlu Y., Kerimoğlu G., Teoman A. S. , Özer Yaman S., ...More

BIOTECHNIC & HISTOCHEMISTRY, vol.95, no.4, pp.317-322, 2020 (Peer-Reviewed Journal) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 95 Issue: 4
  • Publication Date: 2020
  • Doi Number: 10.1080/10520295.2019.1695947
  • Journal Name: BIOTECHNIC & HISTOCHEMISTRY
  • Journal Indexes: Science Citation Index Expanded, Scopus, Academic Search Premier, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, EMBASE, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database
  • Page Numbers: pp.317-322
  • Keywords: endoplasmic reticulum, ethyl pyruvate, ischemia, oxidative stress, rat, reperfusion, stress, testicle, testicular detorsion, ISCHEMIA-REPERFUSION INJURY, N-ACETYLCYSTEINE, TURKISH PROPOLIS, APOPTOSIS, DAMAGE

Abstract

We investigated the effects of ethyl pyruvate (EP) on oxidative and endoplasmic reticulum (ER) stress due to experimental testicular ischemia-reperfusion (I-R). Eighteen rats were divided into a control group, a torsion-detorsion (T-D) group and an EP group. For pretreatment of the EP group, 50 mg/kg EP was given intraperitoneally (i.p.) 30 min before detorsion. Tissue 4-hydroxynonenal (4-HNE) and 78-kDa glucose-regulated protein (GRP78) levels were determined using enzyme-linked immunosorbent assay (ELISA) kits. Tissue total oxidant status (TOS) and total antioxidant status were determined using colorimetric methods. Histology of the tissues was evaluated using hematoxylin and eosin staining. In the T-D group, tissue 4-HNE, GRP78, TOS and oxidative stress index levels were significantly higher than for the control group. The increases were reduced significantly by EP pretreatment. Our findings suggest that EP can inhibit I-R induced testicular injury by suppressing oxidative and ER stress. EP may be a useful adjunctive treatment for surgical repair in humans.