Development of mycoinsecticide formulations with Beauveria bassiana and Metarhizium brunneum for the control of Orosanga japonica (Hemiptera: Ricaniidae)

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Biryol S., Güney E., Eski A., Bayramoğlu Z., Sezen K., Demirbağ Z., ...Daha Fazla

ANNALS OF APPLIED BIOLOGY, cilt.179, sa.3, ss.319-330, 2021 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 179 Sayı: 3
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1111/aab.12699
  • Dergi Adı: ANNALS OF APPLIED BIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Geobase, Veterinary Science Database
  • Sayfa Sayıları: ss.319-330
  • Anahtar Kelimeler: Beauveria, biological control, Metarhizium, oil&#8208, based mycoinsecticide, Orosanga japonica, DESICCATION TOLERANT BLASTOSPORES, ULTRA-VIOLET RADIATION, OIL-BASED FORMULATION, SPRUCE BARK BEETLE, ENTOMOPATHOGENIC FUNGI, ANISOPLIAE CONIDIA, COLEOPTERA, VIRULENCE, PATHOGENICITY, PROTECTION
  • Karadeniz Teknik Üniversitesi Adresli: Evet

Özet

Entomopathogenic fungi are promising microbial agents for agricultural pests, especially piercing-sucking insects. In this study, eight indigenous fungal isolates including four isolates of Metarhizium brunneum and four isolates of Beauveria bassiana were tested on Orosanga japonica Melichar (Hemiptera: Ricaniidae) in order to find an effective control agent that can be utilised for the development of oil-based fungal mycoinsecticides. In the screening test using 1 x 10(7) spores ml(-1) concentration, KTU-24 (B. bassiana) and KTU-51 (M. brunneum) had the highest lethal activity against nymphs and adults of the target pest. Also, at the highest concentration (10(9) spores ml(-1)), KTU-24 showed 92.33 and 94.88% mortality on nymphs and adults at the dose-response tests, respectively, and the KTU-51 isolate showed 100% mortality on both stages of O. japonica under laboratory conditions. With these results, KTU-24 and KTU-51 isolates were used to develop biopesticides. While the mass production of B. bassiana was carried out with liquid-state fermentation, solid-state fermentation was used for mass production of M. brunneum. Spores of both isolates were formulated in oil, and products were named as RICANICIDAL Bbas-TR61 and RICANICIDAL Met-TR61, respectively. Formulations caused over 97% mortality against nymphs and adults of O. japonica under field conditions and both formulations showed higher efficacy than commercial mycoinsecticides at 10(8) spores/ml concentration 20 days after treatment. The currents study shows that both mycoinsecticide formulations are highly promising for management of O. japonica.