Investigation of the Effects of Oleuropein on Mouse Detrusor Muscle Contractility


BARUT E. N., Ozturk A. C., ENGİN S., RENDA G.

Fabad Journal of Pharmaceutical Sciences, vol.49, no.1, pp.81-90, 2024 (Scopus) identifier

  • Publication Type: Article / Article
  • Volume: 49 Issue: 1
  • Publication Date: 2024
  • Doi Number: 10.55262/fabadeczacilik.1358967
  • Journal Name: Fabad Journal of Pharmaceutical Sciences
  • Journal Indexes: Scopus, EMBASE, International Pharmaceutical Abstracts, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.81-90
  • Keywords: Carbachol, detrusor smooth muscle, isolated organ bath, mice, oleuropein
  • Karadeniz Technical University Affiliated: Yes

Abstract

Oleuropein (OLE) is an important bioactive compound isolated from Olea europaea L. (olive), the best-known species of the genus Olea, which grows in the Mediterranean region and has many biological activities. In this study, we aimed to investigate the effect of OLE on isolated mouse detrusor smooth muscle (DSM) contractility. OLE was isolated from Olea europaea L and the effects of OLE on the contractility of DSM strips were investigated using in vitro isolated organ bath systems. The relaxant effect of OLE (10-8-10-3 M) cumulatively on KCl-precontracted strips was evaluated. Moreover, the effect of OLE (10-4 and 10-3 M) incubation on KCl-, carbachol (CCh)- or electrical field stimulation (EFS)-induced contractile responses of detrusor strips were assessed concentration-dependently. OLE at higher concentration (10-3 M) relaxed KCl-precontracted detrusor strips significantly. Moreover, the contractile responses to KCl, CCh, and EFS were significantly decreased in the strips preincubated with OLE (10-3 M) (p<0.05). Our results showed that OLE decreased the contractility of isolated mouse detrusor strips in a concentration-dependent manner and thus it would be a promising agent in pathological conditions related to increased bladder contractility such as overactive bladder. Additionally, further studies are needed to elucidate the exact mechanisms of these effects of OLE.