Protective Effects of Ellagic Acid on Testicular Ischemia-Reperfusion Injury in Rats

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ŞEKERCİ Ç. A., AYDIN H. R., LİVAOĞLU A., YİĞİT E., TOPRAK T., Ramazanoglu M. A., ...More

Journal of Urological Surgery, vol.10, no.2, pp.160-166, 2023 (ESCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 10 Issue: 2
  • Publication Date: 2023
  • Doi Number: 10.4274/jus.galenos.2022.2022.0049
  • Journal Name: Journal of Urological Surgery
  • Journal Indexes: Emerging Sources Citation Index (ESCI), TR DİZİN (ULAKBİM)
  • Page Numbers: pp.160-166
  • Karadeniz Technical University Affiliated: Yes


Objective: This study aimed to investigate the protective effects of ellagic acid on testicular ischemia/reperfusion injury in rats. Materials and Methods: Twenty-one Sprague-Dawley rats were randomly divided into three groups: sham, ischemia/reperfusion (I/R), I/R + ellagic acid (EA). All animals underwent left scrotal exploration. In all groups except the sham group, the left testes were rotated 720 degrees clockwise for 3 h and 3 h reperfusion. 10 mg/kg ellagic acid was administered intraperitoneally to the I/R+E group before reperfusion. Then, the left orchiectomy was performed. The testes underwent biochemical and histological examination. Results: There was a significant difference between the sham and the I/R, I/R+EA groups according to the Cosentino system (p<0.001, p=0.036), and there was no difference between the I/R and IR+EA groups (p=0.319). A significant difference was found between sham and I/R groups according to Johnsen spermatogenesis score (p<0.001), but there was no significant difference between sham and I/R+EA groups (p=0.063). Superoxide dismutase, catalase, malondialdehyde, total oxidant status values were statistically different between I/R and I/R+EA groups (p=0.001, 0.002, 0.002, 0.001 respectively). Conclusion: Ellagic acid has a protective effect against testicular ischemia/reperfusion injury in rats.