Akademik Veri Yönetim Sistemi
7th International Eurasian Conference on Biological and Chemical Sciences (EurasianBioChem 2024), Ankara, Türkiye, 2 - 04 Ekim 2024, ss.769
There is evidence in the literature that the simultaneous use of medicinal drugs and several foods can cause food-drug interactions (1). The genus Asparagus L. includes approximately 300 species, which are mainly distributed in the Mediterranean region, Southern Europe, Anatolia, Asia, Africa, and Europe (2). It is noted that several Asparagus species are used to treat rheumatism, arthralgia, epilepsy, urinary system diseases, stomach ailments, and respiratory system diseases such as bronchitis, pharyngitis, laryngitis, colds, and flu. The edible young shoots of Asparagus officinalis L., known as "kuşkonmaz", are widely consumed as vegetables in salads, meals, and soups (3, 4). Cytochrome P450 (CYP450) is a group of enzymes expressed in different organs, mainly in the liver, and involved in xenobiotic and drug metabolism. Among CYP enzymes, CYP1A1 is responsible for the metabolism of many drugs such as caffeine, theophylline, paracetamol, riociguat, granisetron, axitinib, erlotinib, and conivaptan (3). Upon review of the literature, there are no studies assessing the efficacy of A. officinalis on CYP1A1. The objective of this study was to evaluate the activity of methanol and ethyl acetate extracts obtained from the young shoots of A. officinalis on CYP1A1. The effects of 5 mg/mL doses of methanol and ethyl acetate extracts prepared from young shoots of A. officinalis on CYP1A1 enzyme activities in rat brain microsomes were investigated using the 7-Ethoxyresorufin Odeethylase (EROD) assay. EROD activity is measured by observing the CYP1A1-mediated deethylation of the substrate 7-ethoxyresorufin to generate the product resorufin, which can be fluorometrically monitored. Caffeine was used as a standard, whereas dimethyl sulfoxide, in which the extracts were dissolved, was chosen as a control. Methanol and ethyl acetate extracts from young shoots of A. officinalis inhibited CYP1A1 enzyme activity by 81% and 73%, respectively (caffeine: 63%). The study predicted that A. officinalis, which is widely consumed as food, affects CYP1A1 activity. A. officinalis should be carefully consumed with drugs metabolized by the CYP 1A1 isozyme.
Keywords: food-drug interaction, Asparagus officinalis L., EROD assay, CYP 1A1.