Amsacta moorei entomopoxvirus encodes a functional DNA photolyase (AMV025)

Nalcacioglu R., Dizman Y. A., VLAK J. M., DEMİRBAĞ Z., van Oers M. M.

JOURNAL OF INVERTEBRATE PATHOLOGY, cilt.105, sa.3, ss.363-365, 2010 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 105 Sayı: 3
  • Basım Tarihi: 2010
  • Doi Numarası: 10.1016/j.jip.2010.06.013
  • Dergi Adı: JOURNAL OF INVERTEBRATE PATHOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.363-365
  • Karadeniz Teknik Üniversitesi Adresli: Evet

Özet

The major damage induced in DNA by ultraviolet light is the induction of cyclobutane pyrimidine dimers (CPDs). Amsacta moorei entomopoxvirus (AMEV) encodes a CPD photolyase (AMV025) with a putative role in converting these dimers back into monomers. In infected Lymantria dispar cells transcription of the AMV025 gene started 8 h post inoculation (p.i.) and continued through 38 h p.i. Transcription was inhibited by a DNA synthesis blocker. Transient expression in an Escherichia coli strain that lacks its endogenous photolyase, rescued growth of the UV-irradiated bacteria in a light-dependent manner, showing that AMV025 encodes a functional DNA photolyase. (C) 2010 Elsevier Inc. All rights reserved.