Insecticidal activities of wild type and recombinant invertebrate iridescent viruses on five common pests

Gencer D., Yesilyurt A., Gullu M., DEMİR İ., NALÇACIOĞLU R.

TURKIYE ENTOMOLOJI DERGISI-TURKISH JOURNAL OF ENTOMOLOGY, vol.44, no.3, pp.365-373, 2020 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 44 Issue: 3
  • Publication Date: 2020
  • Doi Number: 10.16970/entoted.700189
  • Journal Name: TURKIYE ENTOMOLOJI DERGISI-TURKISH JOURNAL OF ENTOMOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, CAB Abstracts, Veterinary Science Database, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.365-373
  • Keywords: Invertebrate iridescent virus, insecticidal activity, recombinant virus, TENEBRIO-MOLITOR, NEUROTOXIN, IRIDOVIRUSES, CONSTRUCTION, INFECTION, APOPTOSIS
  • Karadeniz Technical University Affiliated: Yes

Abstract

Invertebrate iridescent virus 6 (IIV6) can infect a broad range of pest insect species. Viruses with new features created by recombinant DNA technology can be used effectively as biological control agents. Previously, recombinants have been constructed: IIVs harboring green fluorescent protein gene (gfp) in place of IIV6 157L ORF (rCIV-Delta 157L-gfp) and a scorpion Androctonus australis (Linnaeus, 1758) insect toxin gene (AaIT) fused with gfp (rCIV-Delta 157L/gfp-AaIT). In this study, wild type IIV6 and the two recombinants, were evaluated for their ability to cause infections on Helicoverpa armigera (Hubner, 1805) (Lepidoptera: Noctuidae), Spodoptera littoralis (Boisduval, 1883) (Lepidoptera: Noctuidae), Lymantria dispar (Linnaeus, 1758) (Lepidoptera: Erebidae), Euproctis chtysorrhoea (Linnaeus, 1758) (Lepidoptera: Erebidae) and Tenebrio molitor (Linnaeus, 1758) (Coleoptera: Tenebrionidae) larvae. This study was performed at Karadeniz Technical University, Department of Biology during 2018 and 2019. Five different concentrations (10(3), 10(4), 10(5), 10(6) and 10(7) TCID50/ml) of viruses were used to inject each insect larvae. All larvae, infected with rCIV-Delta 157L/gfp-AaIT, became paralyzed, except S. littoralis. The LC50 of insect larvae infected by rCIV-Delta 157L/gfp-AaIT were determined as 0.3 x 10(7), 0.7 x 10(5), 0.2 x 10(5), 0.15 x 10(5), 0.7 x 10(4) TC1D(50)/ml on S. littoralis, T. molitor, L. dispar, H. amiigera and E. chtysorrhoea, respectively. LT50 values, calculated according to the highest virus concentrations, were found as 10.5, 6.2, 4.7, 7.5 and 5 d on S. littoralis, T. molitor, L. dispar, H. armigera and E. chrysorrhoea, respectively, for rCIV-Delta 157L/gfp-AaIT. This study showed that recombinant IIV6 has increased pathogenicity on some insects from Lepidoptera and Coleoptera.