Isolation of pathogenic bacteria of the predator beetle Calosoma sycophanta

Yaman M., Ayar Ö., Güner B. G., Ertürk Ö., EROĞLU M.

Revista de Silvicultura ?i Cinegetica, vol.23, no.43, pp.9-12, 2018 (Peer-Reviewed Journal)

  • Publication Type: Article / Article
  • Volume: 23 Issue: 43
  • Publication Date: 2018
  • Journal Name: Revista de Silvicultura ?i Cinegetica
  • Journal Indexes: CAB Abstracts
  • Page Numbers: pp.9-12
  • Karadeniz Technical University Affiliated: Yes


Abstract : Calosoma sycophanta L. (ColeopteraCarabidae) is reared in insect rearing laboratories and released to forest infested by Thaumetopoea pityocampa (Den. & Schiff.). However, this predator is infected by microorganisms. This is the main factor effecting mast production of C. sycophanta. In this study, the bacterial pathogens of Calosoma sycophanta in the rearing laboratories in the years 2015-2017 were studied. During three years 302 bacterial isolates were isolated and identified using VITEK bacterial identification systems. All pathogens were recorded from the predatory beetle in the rearing laboratories for the first time. The majority of identified species were entomopathogenic species. Six most isolated bacteria were further identified using 16SrDNA analysis and tested for insecticidal potential on the larvae and adults of C. sycophanta. All of them were found to be pathogenic on the adults or larvae. Streptomyces albogriseolusProteus penneri have 33.33% and 29.52% mortality on the C. sycophanta adults, respectively. S. albogriseolus, P. penneri, P. vulgaris, P. putida, P. rettgeri and P. vermicola have 29.41%, 26.66%, 40%, 24%, 20% and 20% mortality on the C. sycophanta larvae, respectively. Results show that the rearing laboratories are under the risk of bacterial diseases in the term of mass production of C. sycophanta.