Calcium homeostasis in cisplatin resistant epithelial ovarian cancer

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Kucukkaya B., Basoglu H., Erdag D., AKBAŞ F., SÜSGÜN S., Yalcintepe L.

GENERAL PHYSIOLOGY AND BIOPHYSICS, cilt.38, sa.4, ss.353-363, 2019 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 38 Sayı: 4
  • Basım Tarihi: 2019
  • Doi Numarası: 10.4149/gpb_2019013
  • Dergi Adı: GENERAL PHYSIOLOGY AND BIOPHYSICS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.353-363
  • Anahtar Kelimeler: Drug resistance, MDAH-2774, Cisplatin, Calcium, INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR, ENDOPLASMIC-RETICULUM STRESS, CELL-DEATH, BH4 DOMAIN, CA2+ ENTRY, BCL-2, APOPTOSIS, RELEASE, INHIBITION, PROTEIN
  • Karadeniz Teknik Üniversitesi Adresli: Hayır

Özet

Intracellular calcium concentration ([Ca2+](i)) may have an important role in the development of chemoresistance, which is an essential problem in cancer chemotherapy. Cisplatin (DDP), which modulates the intracellular calcium concentration by different mechanisms, is an antineoplastic agent with high success rate in cancer therapies. We investigated the regulatory role of [Ca2+]in cisplatin resistance in epithelial ovarian cancer cell line, in MDAH-2774, and its chemoresistant subclone MDAH-2774/DDP. The measurement of [Ca2+](i) using fluorescence microscope, and flow cytometry revealed that the amount of intracellular calcium decreased in cisplatin resistant cells compared to the amounts in parental cells. mRNA expression profiles of calcium homeostasis-associated major genes (IP(3)R1/2/3, RYR1/2, SERCA1/2/3, NCX1/2/3, PMCA1/2/3, and PMCA4) decreased in cisplatin resistant cell line in comparison to the expression profiles in parental cells. Owing to the changes in the expression of genes involved in calcium regulation, these results show, drug resistance may be prevented by introducing a new perspective on the use of inhibitors and activators of these genes, and thus of cytostatic treatment strategies, due to changes in the expression of genes involved in calcium regulation.