In vitro biological properties of chitosan and polydopamine-based biopolymer surfaces fabricated on AZ61L alloys
SCIENTIFIC REPORTS, cilt.1, sa.1, ss.1-8, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 1 Sayı: 1
- Basım Tarihi: 2026
- Doi Numarası: 10.1038/s41598-026-59820-0
- Dergi Adı: SCIENTIFIC REPORTS
- Derginin Tarandığı İndeksler: Academic Search Ultimate (EBSCO), Natural Science Collection (ProQuest), Biological Science Database (ProQuest), Biomedical Reference Collection: Corporate Edition (EBSCO), Health Research Premium Collection (ProQuest), Scopus, Science Citation Index Expanded (SCI-EXPANDED), BIOSIS, Chemical Abstracts Core, EMBASE, MEDLINE, Directory of Open Access Journals, Zoological Record
- Sayfa Sayıları: ss.1-8
- Karadeniz Teknik Üniversitesi Adresli: Evet
Özet
In this study, chitosan, polydopamine and polydopamine/chitosan coatings were coated on AZ61L magnesium by dip coating process. Thus, the surface characteristics, wettability and in vitro biological properties including bacteria and cell behavior of chitosan (Mg-C), polydopamine (Mg-D), polydopamine/chitosan (Mg-C-D) layers fabricated on AZ61L alloy were investigated in this work. The surfaces were characterized by X-ray diffractometer, scanning electron microscope, energy dispersive spectrometer, contact angle goniometer and surface profilometer. The Mg-D and Mg-C coatings indicated hydrophilic behavior while the Mg-C-D coatings exhibited hydrophobic character. Cell viability was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, while cell death was confirmed by acridine orange/propidium iodide fluorescence staining. The results demonstrated that all coated surfaces significantly improved cellular responses compared to bare Mg, with the Mg-C-D coating exhibiting the highest cell viability and enhanced cytocompatibility. Antibacterial activity was determined using the agar diffusion method against Staphylococcus aureus (ATCC6538). Furthermore, antibacterial tests revealed that Mg-C-D surfaces showed superior inhibition against bacterial growth compared to single-layer coatings, indicating a synergistic effect between chitosan and polydopamine. These findings demonstrate that the combined coating strategy provides an effective approach to enhance both biocompatibility and antibacterial performance of Mg-based alloys for biomedical applications.