Exogenous ascorbic acid mitigates accumulation of abscisic acid, proline and polyamine under osmotic stress in maize leaves

TERZİ R., KALAYCIOGLU E., DEMIRALAY M., SAĞLAM A., KADIOĞLU A.

ACTA PHYSIOLOGIAE PLANTARUM, cilt.37, sa.3, 2015 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 37 Sayı: 3
  • Basım Tarihi: 2015
  • Doi Numarası: 10.1007/s11738-015-1792-0
  • Dergi Adı: ACTA PHYSIOLOGIAE PLANTARUM
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Anahtar Kelimeler: Ascorbic acid, Abscisic acid, Maize, Proline, Polyamine metabolism, Osmotic stress, WATER-DEFICIT STRESS, OXIDATIVE STRESS, DROUGHT STRESS, SALT STRESS, FOLIAR APPLICATION, PROTECTIVE ROLE, ABIOTIC STRESS, RESPONSES, PLANTS, ARABIDOPSIS
  • Karadeniz Teknik Üniversitesi Adresli: Evet

Özet

The effects of osmotic stress (polyethylene glycol, PEG 6000) and exogenous ascorbic acid (ASC) on abscisic acid (ABA), some osmolytes (proline and polyamine), and gene expression of polyamine metabolic enzymes arginine decarboxylase (ADC), S-adenosylmethionine decarboxylase (SAMDC) and polyamine oxidase (PAO1) were assessed in the leaves of Zea mays L. seedlings. ASC (0.1 mM) was hydroponically applied to detached maize seedlings. ASC application scavenged endogenous hydrogen peroxide level and ameliorated leaf water status and stomatal conductance under osmotic stress. Lipid peroxidation, ABA and osmolyte accumulation were mitigated by ASC treatment. For osmolytes, the alleviation of polyamine accumulation was more obvious than that of the proline. Therefore, gene expressions of polyamine metabolic enzymes were investigated. Compared to control seedlings, down-regulation of SAMDC and ADC gene expressions were determined in ASC ? PEG-applied seedlings while PAO1 gene expression increased. These results indicated that exogenous ASC influenced polyamine metabolism. ASC application under the osmotic stress ameliorated osmotic solute levels and ABA concentration in maize.