Gram stain-negative, motile, catalase-, and oxidase-positive strain, designated DSHK107T was isolated from soil of Cukurova University campus in Adana, Turkey. Its taxonomy was investigated using a polyphasic approach. The strain grew at 20-42 degrees C (optimum, 30 degrees C), at pH 6.0-9.0 (optimum, pH 7.0) and in 0-2.0% NaCl (w/v). 16S rRNA gene sequence analysis revealed that the strain belonged to the genus Fontibacillus; strain DSHK107T showed highest sequence similarity to type strains Fontibacillus aquaticus GPTSA 19T (97.8%) and Fontibacillus panacisegetis P11-6T (97.0%). The major fatty acid of strain DSHK107T was anteiso-C15: 0 (46.7). The polar lipids of strain DSHK107T consisted of dihosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, four unknown phospholipids, four unknown lipids, three unknown glycolipids, two unknown aminophospholipids, an unknown aminolipid, and an unknown aminophosphoglycolipid. The major isoprenoid quinone was MK-7. The DNA G_C content of DSHK107T was 42.1 mol%. DNA-DNA hybridization showed that the strain DSHK107T shared low DNA-DNA relatedness with F. aquaticus DSM 17643T, Fontibacillus solani A4STRO4T, and F. panacisegetis DSM 28129T (47, 58, and 59.3%, respectively). Thus, our results support the placement of strain DSHK107T within a separate and previously unrecognized species. On the basis of a taxonomic study using a polyphasic approach, strain DSHK107T is considered to represent a novel species of the genus Fontibacillus, for which the name Fontibacillus pullulanilyticus sp. nov. is proposed. The type strain is DSHK107T (=NCCB 100560(T)=DSM 100116T).